Plus reactions worked differently: they used T4 DNA polymerase, an enzyme with strong 3’ to 5’ exonuclease activity, meaning it can chew back the end of a DNA strand. In the presence of only one dNTP, T4 DNA polymerase would degrade each fragment from its 3’ end until it reached a nucleotide complementary to that dNTP, at which point the exonuclease activity would be inhibited. This ensured that all fragments in a given plus reaction ended with the same nucleotide.
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“推动建立行业自律技术服务平台，是我国核电在国际上从‘并跑’迈向‘领跑’、实现高质量发展的关键支撑。”卢铁忠委员认为，推动核电从“跟跑”“并跑”迈向“领跑”，必须在运行管理、绩效评价、技术标准等方面建立自主话语体系。